Abstract
BackgroundThe determination of sensitive chemotherapy drugs for gastric cancer (GC) is one of the greatest challenges of adjuvant therapy. Here we evaluated the chemosensitivity of GC to anticancer drugs and the telomerase reverse transcriptase (hTERT) mRNA expression, and investigated the relationship of them.MethodsThe GC cells which were collected from 68 patients with primary GC were primary cultured. The chemosensitivity of GC cells to anticancer drugs was evaluated successfully using the MTT assay for 60 cases of GC cells, and the hTERT mRNA expression was examined in 60 cases of GC tissues and corresponding normal gastric mucosa and 6 cases of chronic superficial gastritis mucosa by in situ hybridization.ResultsTaxol, Cisplatin and 5-Fluorouracil were in general more effective than Adriamycin and Mitomycin for GC cells, and the chemosensitivity to anticancer drugs was associated with tumor histological types and a worse tumor grade. Compared to normal gastric mucosa tissues, hTERT mRNA expression was significantly increased in GC (P<0.05), which was related with a worse differentiation and drug-resistance to 5-Fluorouracil or Adriamycin in GC.ConclusionsThese data demonstrate for the first time that examinations of hTERT mRNA expression as an important factor could be used to select the chemotherapeutic drugs for GC patients.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1793217009875483
Highlights
The incidence of gastric cancer (GC) has been significantly reduced in developed coutries, GC remains one of the most common malignancies worldwide and ranks second in terms of global cancer-related mortality [1,2]
Chemotherapeutic regimens for cancer patients usually are based on the statistical results of clinical trials or the histological type of tumor rather than the cellular sensitivity to each anticancer drug [3,4,5]
HTERT expression could be used to evaluate the efficacy of anticancer drugs
Summary
The incidence of gastric cancer (GC) has been significantly reduced in developed coutries, GC remains one of the most common malignancies worldwide and ranks second in terms of global cancer-related mortality [1,2]. Evaluation of the effectiveness of an anticancer drug prior to Telomeres at the termini of each chromosome, which consist of TTAGGG repetitive DNA sequences and various binding proteins, have an important role in regulating the life-span of human cells and are synthesized by telomerase as a RNA-dependent DNA polymerase [9,10,11]. Most of normal human somatic cells lack telomerase activity due to the stringent transcriptional repression of the hTERT gene. They thereby progressively lose their telomeres with each cell division and are eventually triggered to undergo senescence (irreversible cell growth arrest) when their telomeres become extremely short [13,14]. We evaluated the chemosensitivity of GC to anticancer drugs and the telomerase reverse transcriptase (hTERT) mRNA expression, and investigated the relationship of them
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