Abstract
We examined the localization of basic fibroblast growth factor (bFGF) in a series of human breast carcinomas using immunohistochemistry. Staining was observed in tumour cells in 15 out of 54 (28%) tumours and in the adjacent stroma in 34 out of 54 (63%) tumours examined. No correlation was observed between positive staining of these two compartments. The relationship between bFGF staining and expression of the metalloprotease stromelysin-3, and between bFGF and microvessel density, was examined. A statistically significant correlation (P < 0.003) was observed between bFGF staining of the stromal compartment and high expression of stromelysin-3 (ST-3; MMP-11) metalloprotease mRNA by stromal cells. In contrast, no correlation was observed between bFGF and intratumour microvessel density (IMD). These results raise the possibility that bFGF may be involved in the induction of stromelysin-3 mRNA expression in breast cancer stroma.
Highlights
Formalin-fixed sections were immunostained with antibodies to basic fibroblast growth factor using the peroxidase technique
In 15 tumours (27.8%), basic fibroblast growth factor (bFGF) immunoreactivity was observed in neoplastic cells. bFGF immunoreactivity in the tumoural stroma was observed in 34 cases (62.9%)
The association was limited to stromal bFGF staining and ST3 expression, whereas tumour cell bFGF staining was not associated with ST-3 expression
Summary
The aim of the present study was to examine the significance of immunohistochemical staining of bFGF in human breast carcinomas
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