Expression and Significance of Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor and Transforming Growth Factor β1 in Non-small Cell Lung Cancer

Abstract

Objective To evaluate the expression of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF)and transforming growth factor betal(TGFβ1)in non-small cell lung cancer (NSCLC)and to investigate its relationship to histopathological features.Methods The expression of VEGF,bFGF,TGFβ1 and CD34 were examined in 50 specimens of NSCLC and 18 specimens of para-cancerous tissue by using SP immunohistochemistry staining.Assessment of microvessel density(MVD)was performed.Results The positive rate of VEGF,bFGF,TGFβ1 was 68%.The expression of VEGF was related to clinical classification and lymph nodes metastasis.The difference was significant among the groups(χ2=4.535 and 4.235,P＜0.05).The expression of VEGF had no significant relationship with some pathological characteristics such as histological types and pathological grade.The positive rate of bFGF was 58%.The expression of bFGF was related to clinical classification and lymph nodes metastasis.The difference was significant among the groups (χ2=3.864 and 3.876,P＜0.05).The expression of bFGF has no significant relationship with some pathological characteristics such as histological types,pathological grade.The positive rate of TGFβ1 was 60%.The expression of TGFl3l had significant relationship with some pathological characteristics such as histological types,clinical classification and lymph nodes metastasis.The difference was significant among the groups (χ2=3.852,4.151 and 4.443,P＜0.05).The expression ofTGFβ1 was not related to pathological grade.The expression of MVD was related to clinical classification and lymph nodes metastasis.The difference was significant among the groups(t=2.312 and 2.865,P＜0.05).The expression of MVD had 110 significant relationship with some pathological characteristics such as histological types and pathological grade.The four of all factors had closed statistical significance between cancerous tissue and para.cancerous tissue.The expressions of VEGF and bFGFwere related to MVD.The difference Was significant among the groups(t=2.518 and 3.679,P＜0.05).The expression of TGFl3,had no significant relationship with MVD.Conclusion VEGF,bFGF and TGFDl have very high expressions in NSCLC.The expression levels of VEGF,bFGF and TGFβ1 are significantly related to the invasive growth and metastasis of NSCLC.VEGF and bFGF play an important role during the tumor neovascularization and tumor metastasis.TGFβ1 may be associated with the decreasing of immunoregulation function and the escape of immune survival. Key words: NSCLC; VEGF; bFGF; TGFβ1; MVD