Abstract
The arms race between plants and viruses never ceases. Chinese cabbage, an important type of Brassica vegetable crop, is vulnerable to plant virus infection, especially to Turnip mosaic virus (TuMV). To better examine the molecular mechanisms behind the virus infection, we conducted the correlation analysis of RNA-Seq and quantitative iTRAQ-LC-MS/MS in TuMV-infected and in healthy Chinese cabbage leaves. There were 757 differentially expressed genes and 75 differentially expressed proteins that were screened in Chinese cabbage plants infected with TuMV. These genes were enriched in many pathways, and among them, the plant hormone signal transduction, plant-pathogen interaction, and protein processing in the endoplasmic reticulum pathways were suggested to be closely related pathways. The correlation analysis between RNA-Seq and quantitative iTRAQ-LC-MS/MS was then further explored. Finally, we obtained a preliminary network of several candidate genes associated with TuMV infection, and we found that they mainly belonged to calcium signaling pathways, heat shock proteins, WRKY transcription factors, and non-specific lipid transfer proteins. These results may lead to a better understanding of antiviral mechanisms and of disease-resistant breeding.
Highlights
Brassica vegetable crops provide multiple components of nutrition such as proteins, vitamins, trace elements, and inorganic salts, and serve as important research materials
Based on the transcriptome and proteome data, we summarized the candidate genes and pathways related to Turnip mosaic virus (TuMV) infection on a preliminary network (Figure 7) that mainly focused on calcium signaling pathways, heat shock proteins (HSPs), WRKY transcription factors, and non-specific lipid transfer proteins
Plant Hormones Are Correlated to TuMV Resistance
Summary
Brassica vegetable crops provide multiple components of nutrition such as proteins, vitamins, trace elements, and inorganic salts, and serve as important research materials. Turnip mosaic virus (TuMV), a member of the Potyvirus genus, has emerged as an important pathogen to Brassica crops (Walsh and Jenner, 2002). TuMV Resistance of Chinese Cabbage virus (CMV) and is the most prevalent viral pathogen infecting Chinese cabbage, especially in Asia, North America, and Europe (Tomlinson, 1987). It infects 318 kinds of dicotyledons and several monocotyledons through artificial inoculation (Walsh and Jenner, 2002; Shattuck, 2010). Investigation of natural plant genes that confer resistance against viruses may provide effective and environmentally friendly ways for the reduction or restriction of viral infections (Fraser, 1990; Hughes et al, 2002; Walsh and Jenner, 2002; Li et al, 2019)
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