Abstract
Corneal scarring limits vision for millions of individuals worldwide. Corneal transplantation (keratoplasty) is the standard of care for corneal opacity; however, it bears the risk of graft rejection and infection and is not universally available. Stem cell therapy holds promise as an alternative to keratoplasty. Stem cells from human corneal stroma (CSSC) induce regeneration of transparent corneal tissue in a mouse wound-healing model. In this study we investigated the mechanism by which CSSC prevent deposition of fibrotic tissue. Infiltration by CD11b+/Ly6G+ neutrophils and myeloperoxidase expression were increased in corneas 24 hr after corneal wounding but were reduced in CSSC-treated wounds. Secretion of TSG-6, a protein known to regulate neutrophil migration, was up-regulated in CSSC in response to TNFα and as CSSC differentiate to keratocytes. In vivo, wounded mouse corneas treated with CSSC contained human TSG-6. Inhibition of neutrophil infiltration into cornea by CSSC was reversed when TSG-6 expression was knocked down using siRNA. Silencing of TSG-6 expression in CSSC reduced their ability to block scarring and the expression of mRNA for fibrosis-associated proteins collagen III, tenascin C, and smooth muscle actin in wounded corneas. Neutropenic mice exhibited a significant reduction in corneal scarring and fibrotic mRNA expression 2 weeks after wounding. These results support the conclusion that neutrophil infiltration is an essential event in the fibrotic response to corneal damage and that prevention of scarring by CSSC is mediated by secretion of TSG-6 by these cells.
Highlights
Corneal blindness resulting from ocular trauma or infection affects 7–10 million people worldwide[1]
A study from this laboratory showed that corneal stromal stem cells (CSSC) in fibrin gel engraft into the mouse cornea and prevent corneal scarring in response to corneal debridement wounds[12]
We found that CSSC engrafted into the wound reduced the number of neutrophils present in the cornea 24 hr after wounding
Summary
Corneal blindness resulting from ocular trauma or infection affects 7–10 million people worldwide[1]. The only treatment option for most of these individuals consists of corneal transplantation (lamellar or penetrating keratoplasty), a procedure complicated by tissue rejection and limited by the supply of donor tissue[2]. There is an increasingly important need to develop alternative therapies for these patients. A role for neutrophils in corneal scarring. JLF; Research to Prevent Blindness, Inc. Unrestricted Grant for Vision Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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