Corneal endothelium transports fluid in the absence of net solute transport

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The corneal endothelium transports fluid from the corneal stroma to the aqueous humor, thus maintaining stromal transparency by keeping it relatively dehydrated. This fluid transport mechanism is thought to be driven by the transcellular transports of HCO 3 − and Cl − in the same direction, from stroma to aqueous. In parallel to these anion movements, for electroneutrality, there are paracellular Na + and transcellular K + transports in the same direction. The resulting net flow of solute might generate local osmotic gradients that drive fluid transport. However, there are reports that some 50% residual fluid transport remains in nominally HCO 3 − free solutions. We have examined the driving force for this residual fluid transport. We confirm that in nominally HCO 3 − free solutions, 48% of control fluid transport remains. When in addition Cl − channels are inhibited, 30% of control fluid movement still remains. Addition of a carbonic anhydrase inhibitor has no further effect. These manipulations combined inhibit the transcellular transport of all anions, without which there cannot be any net transport of solute and consequently no local osmotic gradients, yet there is residual fluid movement. Only the further addition of benzamil, an inhibitor of epithelial Na + channels, abolishes fluid transport completely. Our data are inconsistent with transcellular local osmosis and instead support the paradigm of paracellular fluid transport driven by electro-osmotic coupling.