Abstract
Here we report on the role of α-catenin in the cellular localization of activated leukocyte cell adhesion molecule, ALCAM, and cadherin-mediated cell adhesion in human prostate cancer cells. Cell lines that have a functional E-cadherin-mediated cell adhesion (DU-145 and LNCaP) show ALCAM staining at cell–cell contacts. In contrast, in cell lines that lack α-catenin expression (ALVA-31, PC-3, and PPC-1), E-cadherin-mediated adhesion is disturbed and ALCAM staining is cytoplasmic. A role of α-catenin in the recruitment of E-cadherin and ALCAM to cell–cell contacts was established by transfection of an α-N-catenin construct into cell lines ALVA-31 and PC-3. This resulted not only in the correct assembly of E-cadherin/α-catenin complexes at the cell membrane but also in localization of ALCAM to cell–cell contacts, indicating that indeed α-catenin affects ALCAM localization.
Published Version
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