Abstract

The conversion of tryptophan to indoleacetamide in the presence of pyridoxal phosphate, horseradish peroxidase and Mn 2+ ions goes through an electronically excited intermediate. This result which was expected on the basis of similarity of the reaction to well established bioluminescent processes, is supported by: (i) photon emission (ii) correlation between total light emission and products formation (iii) formation of pyridoxoic acid.

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