Abstract
A simple mathematical model of the correlation between capillary zone electrophoresis (CZE) and continuous free-flow zone electrophoresis (FFZE) was developed. The model results from the fact that both methods are based on the same separation principle (zone electrophoresis) and both are performed in a free solution of the same composition. Based on this model, a procedure for the conversion of an analytical microscale CZE separation into a preparative FFZE separation was developed. The applicability of this procedure is demonstrated by CZE analysis at the picomole level and FFZE preparative fractionation (60 mg/h throughput) of crude synthetic biologically active peptide [ d-Tle 2,5]-dalargin. The combination of CZE and FFZE provides an efficient and economical tool for synthetic peptide analysis and preparation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.