Abstract
Understanding soil microbial communities in agroecosystems has the potential to contribute to the improvement of agricultural productivity and sustainability. Effects of conversion from long-term wheat plantation to Jerusalem artichoke (JA) plantation on soil fungal communities were determined by amplicon sequencing of total fungal ITS regions. Quantitative PCR and PCR-denaturing gradient gel electrophoresis were also used to analyze total fungal and Trichoderma spp. ITS regions and Fusarium spp. Ef1α genes. Results showed that soil organic carbon was higher in the first cropping of JA and Olsen P was lower in the third cropping of JA. Plantation conversion changed soil total fungal and Fusarium but not Trichoderma spp. community structures and compositions. The third cropping of JA had the lowest total fungal community diversity and Fusarium spp. community abundance, but had the highest total fungal and Trichoderma spp. community abundances. The relative abundances of potential fungal pathogens of wheat were higher in the wheat field. Fungal taxa with plant growth promoting, plant pathogen or insect antagonistic potentials were enriched in the first and second cropping of JA. Overall, short-term conversion from wheat to JA plantation changed soil fungal communities, which is related to changes in soil organic carbon and Olsen P contents.
Highlights
Trichoderma spp. are opportunistic, avirulent plant symbionts, as well as being parasites of other fungi, and can exert beneficial effects on plants through inhibiting plant pathogens and promoting plant growth[20]
Triplicate bulk soil samples were taken from a long-term cultivated wheat field and fields converted to Jerusalem artichoke (JA) plantation for one, two and three years, respectively
Trichoderma and Fusarium spp. community diversities and abundances were determined by analyzing total fungal and Trichoderma spp
Summary
Trichoderma spp. are opportunistic, avirulent plant symbionts, as well as being parasites of other fungi, and can exert beneficial effects on plants through inhibiting plant pathogens and promoting plant growth[20]. The effects of conversion from long-term cultivated wheat field to JA plantation on soil total fungal, Trichoderma and Fusarium spp. communities were analyzed. Structure and composition were analyzed by quantitative PCR (qPCR), PCR-denaturing gradient gel electrophoresis (PCR-DGGE), and Illumina Miseq sequencing. We hypothesize that soil microbial communities differ between wheat and JA fields since land use conversion can induce changes in plant-derived carbon resources. Plants have species-specific effects on soil microbial communities, which may strengthen over time[21]; and successive monocropping can adversely affect soil biodiversity[22,23,24].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
