Convergent solid-phase peptide synthesis. XI. Synthesis and purification of protected peptide segments spanning the entire sequence of the uteroglobin monomer using the photolabile nbb-resin.

Abstract

Protected peptide segments corresponding to the 1–4, 5–11, 19–30, 39–49 and 50–58 segments of the Uteroglobin monomer have been synthesised on a solid support using the photolabile ortho-nitrobenzyl unit as a handle. Photolytic detachment of the peptides from the solid support occurred in good yields. The 50–58 protected peptide segment has also been synthesised using the base-labile NPE handle, and detached from the solid support in excellent yield by treatment with a 20% solution of piperidine in DMF. The protected peptide segments were purified by MPLC and/or preparative HPLC, using solvents containing DMF.