CONVENTIONAL AND MOLECULAR STUDY OF Entamoeba spp. IN DOMESTIC DOG’S FECAL AT BAGHDAD CITY, IRAQ.

A A Faraj,A I Fadhil,S R Fadel

doi:10.36103/ijas.v52i6.1489

Abstract

This study was aimed to detect Entamoeba spp. in domestic dogs by using the microscopic method and PCR. The study includes collection of 100 fecal samples by visiting a small animal private clinics in Baghdad, at the period from January 2019 to February 2020. The microscopical results showed the rate of infection at Entamoeba spp. was (15%). Show a higher infection rate in females (15.55%)) than in males (14.54%)without significant difference (P.0>0.05). The Prevalence of Entamoeba related to age was (22%), (6%) in young and adult age respectively. Molecular examination results, the total infection rate of Entamoeba in dogs fecal showed 15% . Ten positive PCR products were sequenced and deposited in the Genebank database, the first time in Iraq, the phylogenic analysis demonstrated that 10sequences belong to Entamoeba histolytica (MT296770, MT296771, MT296772, MT296773, MT296774, MT296775, MT296776, MT296777, MT296778, and MT296779).

Highlights

‫الع ارق‬، ‫ في ب ارز الكلاب المنزلية في مدينة بغداد‬Entamoeba spp.‫الد ارسة التقليدي والجزيئي لطفيلي‬
For the Amoebiasis, an infection by intestinal protozoa detection of Entamoeba species, the samples belongs to the genus Entamoeba consist of were kept in 5 ml tubes containing 2%
Genus Entamoeba has two forms, the the first one for the direct microscopic trophozoite which has a short life span and is examination using iodine staining, the second mobile that can foray into the different organ part the positive fecal samples with systems, while the cysts are a long-surviving Entamoeba species cysts/trophozoite were form of Entamoeba that colonize the kept at -20 oC for molecular diagnosis (PCR)

Summary

INTRODUCTION

Genus Entamoeba has two forms, the the first one for the direct microscopic trophozoite which has a short life span and is examination using iodine staining, the second mobile that can foray into the different organ part the positive fecal samples with systems, while the cysts are a long-surviving Entamoeba species cysts/trophozoite were form of Entamoeba that colonize the kept at -20 oC for molecular diagnosis (PCR). A few is Genomic DNA estimation known about the molecular diagnosis of the The extracted genomic DNA was measured by species of this parasite in dogs in Iraq. This utilizing a Nanodrop spectrophotometer study determined the prevalence of Entamoeba

MATERIALS AND METHODS

RESULTS

REFRENCES