Abstract
The objective of this study to calculate means of oocysts in feces natural infected sheep. A total of 150 sheep fecal samples collected from different regions of Baghdad province(AL-Sholla , AL-Horia, AL-Taji, and Abo-Grab)-Iraq, during the period from the beginning of January to the end ofMay 2016. Three laboratory methods(modified acid fast stain, sheather᾿s sugar solutionand calculation of oocysts in feces of infected animals by haemocytometer) were used for diagnosis of Cryptosporidial oocysts and study the shedding rate of oocysts from infected male and female(pregnant and non pregnant)animals. The total infection rate was 44.66%(67/150), and the highest infection rate was detected in March, while the lowest infection rate was recorded in January 63%(19/30), 20%(6/30) respectively. Theresults were revealed significance difference between male and female infection rate, 31.74%(20/63), 54.02%(47/87) respectively. The average number of shed oocysts per gram of feces from infected non pregnant ewes was 1440 oocysts per gram while in infected pregnant ewes was 2082 oocysts per gram. The shedding rate of oocysts in pregnant ewes show the highest rate in the end period of pregnancy, while the lowest rate of shedding in the beginning period of pregnancy.
Highlights
Cryptosporidium is an intestinal protozoan parasite that allegedly infects alimentary canal of human and animals, it considered as oneof important zoonotic parasite[21]
The parasite have monoxenous life cycle which mean asexual and sexual stages happen within one host, within 1-3days, the prepatent period varies from 1-3 weeks, whereas the patent periodwhich mean duration of oocysts shedding, can be differs from several days to month, indicating the possibility of this infection continue to persist. [31].Cryptosporidiosis transmitted via the fecal-oral transmission from infected animal to the another or from infected animals to human through contaminated food and water with mature oocysts( 21,32)
This study aimed to investigate the shedding rate of Cryptosporidium oocysts from natural infected sheep
Summary
Cryptosporidium is an intestinal protozoan parasite that allegedly infects alimentary canal of human and animals, it considered as oneof important zoonotic parasite[21]. MATERIALSAND METHODS A total of 150 sheepfecal samples were collected from both sex(87 females and 63 males) from different regions of Baghdad province(AL-Sholla, AL-Horia, AL-Taji, and AboGrab) -Iraq, during the period from January to May 2016. Examination of samples: Three laboratory methods were used to diagnosedCryptosporidial oocysts, and study the shedding rate of oocysts from infected male and female (pregnant and non pregnant), Sheather's sugar solution (Fig: 1) Modified Zeihl-Neelsen Stain (MZN) (Fig: 2) and calculation of Cryptosporidium oocysts in feces of infected sheep by using haemocytometer. RESULTS AND DISCUSSION The results of this study recorded 44.66 % (67/150) of sheep infected with Cryptosporidiosis in different areas of Baghdad city, the highest infection rate in AL-Taji60 % (21/39), while the lowest rate of infection in Abo-Grab 32.5% (13/36) (Table:).This results agreed with Khalil, [26]in Mosul and Al-Gilani, [5] in Baghdad who recorded infection rate in sheep 36.43%, 48.8%respectively, agreed with Al-Kaabi, [6] in Diwaniyah province who found 27.5% of sheep shed Cryptosporidium oocysts, the result agreed with Faleket al,. The differences in infection rates in different regions in the world and in Iraq may be due to differences in the number of animals checked, sample size, climatic conditions, and as wall as different diagnostic methods used in laboratory.[21]
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