Controlled Release Simvastatine from Molecularly Imprinted Cryogel Membranes

Abstract

AbstractIn this study, poly(2‐hydroxyethyl methacrylate‐N‐methacryloyl‐L‐tryptophan methyl ester) cryogel membranes were synthesized in different concentrations by using molecular imprinting technique, for controlled release of simvastatin. The cryogel membranes were prepared in various compositions and thus the releases of cryogels containing 0.5, 0.75 and 1 mg hydrophilic simvstatin were compared. The chemical and morphological properties of the poly(HEMA‐MATrp) cryogel membranes were evaluated through a series of characterization techniques. The membranes were subjected to swelling tests to assess their ability to absorb water and their degree of crosslinking. Fourier‐transform infrared spectroscopy was applied to determine the functional groups present in the cryogel matrix and to evaluate the degree of chemical modification that occurred during synthesis. Additionally, scanning electron microscopy was employed to visualize the cryogel at high resolution, providing insights into the pore structure and overall architecture of the matrix. Then, cryogel membranes were evaluated for cell proliferation abilities by 3‐(4,5‐dimethyl/thiazol‐2‐yl) 2,5‐diphenyltetra zolium bromide thiazolyl blue (MTT) assay for cell viability. The prepared cryogel membranes have a diameter and thickness of about 10 mm and 1.0 mm, respectively. The increasing attention towards the use of cryogel membranes in tissue‐engineering has resulted in demonstrating the dependency of release profiles of simvastatin cryogel membranes on their composition.