Controlled, blinded force platform analysis of the effect of intraarticular injection of autologous adipose-derived mesenchymal stem cells associated to PRGF-Endoret in osteoarthritic dogs

Jose M Vilar,Jose M Carrillo,Angelo Santana,Belen Cuervo,Manuel Morales,Mónica Rubio,Ramón Cugat,Giuseppe Spinella

doi:10.1186/1746-6148-9-131

Abstract

BackgroundAdipose-derived mesenchymal stem cell (ADMSC) therapy in regenerative medicine is a rapidly growing area of research and is currently also being used to treat osteoarthritis (OA). Force platform analysis has been consistently used to verify the efficacy of different therapeutic strategies for the treatment of OA in dogs, but never with AD-MSC.The aim of this study was to use a force platform to measure the efficacy of intraarticular ADMSC administration for limb function improvement in dogs with severe OA.ResultsEight lame dogs with severe hip OA and a control group of 5 sound dogs were used for this study. Results were statistically analyzed to detect a significant increase in peak vertical force (PVF) and vertical impulse (VI) in treated dogs. Mean values of PVF and VI were significantly improved after treatment of the OA groups, reaching 53.02% and 14.84% of body weight, respectively, at day 180, compared with only 43.56% and 12.16% at day 0.ConclusionThis study objectively demonstrated that intraarticular ADMSC therapy resulted in reduced lameness due to OA.

Highlights

Adipose-derived mesenchymal stem cell (ADMSC) therapy in regenerative medicine is a rapidly growing area of research and is currently being used to treat osteoarthritis (OA)
Several experimental strategies have investigated whether mesenchymal stem cells (MSCs), instead of chondrocytes, can be used for the regeneration and maintenance of articular cartilage in OA [3]
Studies and anecdotal clinical experience demonstrate that autologous ADMSC therapy is of clinical benefit in horses and dogs with orthopedic conditions [8,9,10,11,12]

Summary

Introduction

Adipose-derived mesenchymal stem cell (ADMSC) therapy in regenerative medicine is a rapidly growing area of research and is currently being used to treat osteoarthritis (OA). Several experimental strategies have investigated whether mesenchymal stem cells (MSCs), instead of chondrocytes, can be used for the regeneration and maintenance of articular cartilage in OA [3]. Autologous stem cell therapy in the field of regenerative veterinary medicine involves harvesting tissue,such as fat or bone marrow [4], from the patient, isolating the Previous studies evaluating OA therapy in dogs suggest that non-steroidal anti-inflammatory drugs do not often provide complete pain relief [7], perhaps because they rely on a single target receptor or pathway for their action. Studies and anecdotal clinical experience demonstrate that autologous ADMSC therapy is of clinical benefit in horses and dogs with orthopedic conditions [8,9,10,11,12]

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