Abstract
We report a novel engineered microwells to spatially control differentiation of mouse embryoid bodies. With integrating multiple functionally distinct biomaterials by soft-photolithography technology, this method enables simple and reliable manufacture of biochemically heterogeneous microwells that are capable of regulating differentiation of stem cell in a spatial-specific manner. This simple technology offers a new dimension of spatial control over embryoid bodies development and has great potential in tissue engineering and biomedical applications.
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