Control of tectal cell number during larval development in Rana pipiens.

Abstract

Cell production and cell deaths were determined in larval Rana pipiens both in control tecta and in tecta following unilateral eyeball removal in embryos and larvae. Such enucleations produce significantly reduced rates of cell division in the contralateral tecta for virtually the entire larval period (confirming studies with enucleation almost exclusively performed in embryos--Kollros: J. Exp. Zool. 123:153-187, '53, and J. Comp. Neurol. 205:171-178, '82). Significant numbers of cell deaths in all nonependymal tectal cell layers were also observed. Control cell division rates peak at stage X, while cell death peaks are reached in stages XIII-XX. Overall, about 10(6) nonependymal cells are produced in control tecta, and about 350,000 of them die by the end of metamorphosis. Control of cell numbers following enucleation is shown to depend mainly on reductions in cell division rates when the operation occurs early in development and mainly on increases in cell death rates when the operation occurs late in larval life. Such increases in death rates are invariably present within 1 day of the operation whereas the reduced division rates ordinarily require several more days to be seen. The modified rates, both of cell divisions and cell death, are limited to tectal areas to which optic nerve fibers have already extended. Maps of the positions of tectal cell divisions in many larval stages provide the basis for modifying the current dogma that tectal formation occurs as a series of newly formed mediocaudal wedges pushing previously produced wedges rostrolaterad. All such "old" wedges receive substantial cell additions for many stages, with the rate of addition decreasing rostrad earlier than caudad.