Abstract
Kinesin-5 motors fulfill essential roles in mitotic spindle morphogenesis and dynamics as slow, processive microtubule (MT)-plus-end directed motors. The Saccharomyces cerevisiae kinesin-5 Cin8 was found, surprisingly, to switch directionality. Here we have examined Cin8 directionality control using single-molecule fluorescence motility assays and live-cell microscopy. On spindles, Cin8 motors mostly moved slowly towards the midzone, in the plus-end direction of the interpolar MTs. Occasionally, Cin8 also moved faster towards the spindle poles, in the minus-end direction of the MTs.
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