Abstract
The incorporation of cytochrome c oxidase into phospholipid vesicles provides a convenient experimental system for studying the vectorial reactions of the enzyme in isolation from other components of the respiratory chain. One characteristic of the cytochrome c oxidase system in common with mitochondria and submitochondrial particles is that the control of turnover can be demonstrated under appropriate conditions. This chapter presents studies on the effects of ionophores and pH on respiratory control and turnover of cytochrome c oxidase in a reconstituted preparation. The sonication method of Racker used in these studies to prepare reconstituted vesicles of cytochrome c oxidase produces single layered vesicles with a random orientation of cytochrome c oxidase across the membrane. The oxidation of externally added cytochrome c results in an electrogenie production of OH- inside the vesicles. The preparation provides a simple convenient system for studying vectorial reactions catalyzed by membrane bound cytochrome c oxidase. The control of cytochrome c oxidase turnover in the reconstituted system is a consequence of a respiration induced electrical membrane potential.
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