Abstract
The effects of AgNO 3 and ethylene, released by exogenous supplied 2-chloroethylphosphonic acid (Ethephon), were studied on somatic embryogenesis by carrot ( Daucus carota L.) cell suspension cultures. AgNO 3, at concentrations of 10–20 μM, caused a 2-fold increase in the number of somatic embryos. At these concentrations AgNO 3 did not cause adverse effects on cell survival, medium pH or growth; only a slight increase in ethylene production was observed. However, 1 mg·1 −1 or 10 mg·1 −1 Ethephon provoked a substantial decrease in the somatic embryo formation: inhibitory rates of 15% and 50% were recorded, respectively. The activity of arginine decarboxylase (ADC) (EC 4.1.1.19), a key enzyme of the polyamine pathway, was significantly stimulated by AgNO 3 during the first 4 days of somatic embryogenesis, whereas Ethephon reduced it: 30% inhibition was recorded at 10 mg·1 −1 Ethephon. On the basis of these findings, it is suggested that AgNO 3 stimulates carrot somatic embryogenesis by inhibiting ethylene action; its implication in the control of ADC activity is discussed.
Published Version
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