Abstract
Aim: To evaluate the possible roles of phospholipase C, protein kinase C, and endothelin-converting enzyme on contractions induced by ethanol (164 mM) in isolated gastric fundal strips of mice. Materials and methods: After the mice were killed, the stomach of each was removed and longitudinal muscle strips were prepared from the gastric fundus. The strips were mounted in organ baths and their responses were recorded isometrically. Ethanol (164 mM) was added to the organ baths and a steady-state contraction was obtained. After the first ethanol response was recorded, the preparations were incubated separately with lidocaine (1-100 µM), neomycin (10-500 µM), safingol (0.5-5 µM), and SM-19712 (1-50 µM) for 40 min. The second ethanol response was then examined in the presence of the drug used in the incubation period. Results: Lidocaine (1-100 µM), a local anesthetic agent, did not modulate the contractions induced by ethanol in isolated gastric fundal strips of mice. In contrast, neomycin (10-500 µM), a selective inhibitor of phospholipase C, and safingol (0.5-5 µM), a selective inhibitor of protein kinase C, decreased the ethanol-induced contractions. SM-19712 (1-50 µM), a selective inhibitor of endothelin-converting enzyme, failed to affect these contractions. Conclusion: The contractile effect of ethanol may be muscular rather than neuronal in the gastric fundal strips of mice. In addition, phospholipase C and protein kinase C pathways may have a role in contractions due to ethanol in the mouse gastric fundus. On the other hand, endothelin-converting enzyme may not have a regulatory role in the contractile responses of ethanol in the same tissue.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.