Abstract
Abstract Immune stimulation results in draining lymph node swelling and vascular-stromal growth. Lymph node endothelial cell proliferation is upregulated at day 2 after immunization and this early event is dependent on CD11c+ cells and VEGF. Fibroblast-type reticular stromal cells (FRC) are the principal VEGF-expressing cells within lymph nodes. Here, we test the hypothesis that CD11cmed MHCIImed GR1+ cells that accumulate in the draining lymph node plays a role during the upregulation of endothelial cell proliferation at day 2. We show that depletion of GR1+ cells induces a reduction in endothelial cell proliferation in comparison with control mice. In vitro, CD11c+ cells isolated from immunized lymph nodes are able to stimulate VEGF secretion by FRCs. CD11c+Gr1+ cells express IL-1β, and anti-IL-1β treatment reduces lymph node endothelial cell proliferation. Our results suggest that the CD11c+GR1+ cells contribute to the initiation of lymph node vascular growth via the secretion of IL-1β, which then induces increased VEGF expression by FRCs.
Published Version
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