Contrasting dynamics of polychlorinated biphenyl dissipation and fungal community composition in low and high organic carbon soils with biochar amendment.

Abstract

Biochar amendment has been advocated as an effective method to remediate organic pollutant-contaminated soils through adsorption and stimulating microbial degradation. However, such effects can vary depending on soil properties and biochar physiochemical characteristics. The objective of this study was to compare the dynamic variations of polychlorinated biphenyls (PCBs) in low and high soil organic carbon (SOC) soils both amended with biochar and to investigate its linkage with fungal community composition. Two soils having the same texture and soil type but varying in SOC contents were contaminated with PCBs to a final concentration of 60mgkg-1 and amended with 2% bamboo biochar. Temporal changes of PCB remaining in soils and adsorbed on biochar particles were determined during a 2-month incubation. Diversity and composition of fungal communities in both low and high SOC soils were investigated with Illumina MiSeq sequencing. The results showed that the PCB concentrations in low SOC soil were significantly lower than those in high SOC soil during the incubation. In the low SOC soil, the biochar particle adsorbed higher amounts of PCB, tetra-, and penta-chlorobiphenyls (CBs) than those in high SOC soil, and stimulated the dissipation of di- and tri-CBs. The nonmetric multidimensional scaling profile showed significant (p < 0.05) differences in the fungal community composition between the low and high SOC soils. The relative abundances of Eurotiomycetes were gradually increased, whereas those of Sordariomycetes and Dothideomycetes were decreased with increasing incubation time in the low SOC soils. In contrast, the fungal communities in high SOC soils were relatively stable. The relative abundances of Eurotiomycetes and Sordariomycetes were positively correlated with PCB dissipation in low and high SOC content soils, respectively. Our results suggest that SOC content affects PCB dissipation and fungal community composition in biochar-amended soils, and biochars have a high remediation potential of PCB in soils with low SOC contents.