Abstract
The localization and visualization of intact spermatozoa in dried stains confirms the presence of semen in cases of suspected rape. Even though the average human male ejaculate contains over 240 million spermatozoa, current searching methods are often inefficient and tedious. These methods include the light microscopic search for spermatozoa following either: extraction of the stain components in aqueous buffer, destruction of the stain substrate, or the in situ staining of the suspected area.The extraction technique is most commonly employed even though it is inefficient in cell recovery. Spermatozoa apparently bind tenaciously to the support medium during the drying of the seminal plasma. The extraction process often fails in extracting complete cells. The number of spermatozoa collected from a stain may be further reduced by dilution of the semen with other body fluids, dilution of stain components with extraction medium, limited stain size, or a low sperm count due to physiological and/or elective (vasectomy) reasons.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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