Abstract
Sulfadiazine and N4-acetylsulfadiazine were determined in biological fluids by the direct injection (plasma after protein precipitation and urine after dilution 100 times) of 20 μl on a silica gel column. The mobile phase was an aqueous citrate buffer (pH 4.0) and UV detection was at 264nm. Chromatographic selectivity was optimized by the silica gel surface and pH of the mobile phase. Detection limits were ~0.4 μg/ml for sulfadiazine in plasma and ~5 and 7 μg/ml for sulfadiazine and N4-acetylsulfadiazine in urine, respectively. In quantitations by peak heights relative to an internal standard (sulfamerazine), within-run precisions (srel%) for sulfadiazine were 1.7 and 4.0% at 40 and 2 μg/ml, respectively, in plasma and 0.76 and 1.7% at 750 and 25 μg/ml, respectively, in urine.
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