Abstract
In type1 diabetes (T1D) autoreactive T-cells infiltrate the islets of Langerhans, depleting insulin-secreting β-cells (insulitis). Insulitis arises during an asymptomatic phase, prior to clinical diagnosis of T1D. Methods to diagnose insulitis and β-cell mass changes during this asymptomatic phase are limited, precluding early therapeutic intervention. During T1D the islet microvasculature increases permeability, allowing nanoparticles to access the microenvironment. Contrast enhanced ultrasound (CEUS) uses shell-stabilized gas bubbles to provide acoustic backscatter in vasculature. Here, we report that sub-micron sized ‘nanobubble’ ultrasound contrast agents can be used to measure increased islet microvasculature permeability and indicate asymptomatic T1D. Through CEUS and histological analysis, pre-clinical models of T1D show accumulation of nanobubbles specifically within pancreatic islets, correlating with insulitis. Importantly, accumulation is detected early in disease progression and decreases with successful therapeutic intervention. Thus, sub-micron sized nanobubble ultrasound contrast agents provide a predicative marker for disease progression and therapeutic reversal early in asymptomatic T1D.
Highlights
In type[1] diabetes (T1D) autoreactive T-cells infiltrate the islets of Langerhans, depleting insulin-secreting β-cells
We demonstrated that nanobubbles (NBs) accumulate within the pancreas in multiple animal models of type1 diabetes (T1D) as detected by persistent increased ultrasound sub-harmonic contrast, and that histological measurements further localize the accumulation to the infiltrated pancreatic islets
These results suggest that contrast-enhanced ultrasound using NB contrast agents can be used to detect and longitudinally track presymptomatic T1D progression (Fig. 6)
Summary
In type[1] diabetes (T1D) autoreactive T-cells infiltrate the islets of Langerhans, depleting insulin-secreting β-cells (insulitis). Methods to diagnose insulitis and β-cell mass changes during this asymptomatic phase are limited, precluding early therapeutic intervention. Sub-micron sized nanobubble ultrasound contrast agents provide a predicative marker for disease progression and therapeutic reversal early in asymptomatic T1D. Prior to the clinical presentation of diabetes there exists an asymptomatic phase where insulitis and immunological irregularities are present, but there is sufficient β-cell mass and insulin secretion to regulate blood glucose levels. The asymptomatic phase presents an opportunity for therapeutic intervention to blunt insulitis and preserve β-cell mass[4,5]. A method to identify and track the underlying disease progression during the asymptomatic phase is paramount for diagnosing and treating patients at risk for developing T1D. Therapeutic interventions aimed at preventing islet autoimmunity and insulitis and preserving β-cell mass have had limited success. There are limited means to monitor the response to preventative treatments
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.