Abstract
The aim of this study was to observe the effects of Endostar (recombinant human endostatin) and radiotherapy, singly or in combination, on blood flow in mouse tumour tissue using ultrasound imaging. The ultrasound contrast agent, SonoVue, was used for the contrast-enhanced ultrasound examinations. SonoLiver software was used to analyse dynamic vascular patterns (DVPs) in the contrast process. Blood perfusion data were collected and statistical analysis was performed for data processing. Results were presented as DVP curves and quantitative parameters. Quantitative parameters showed statistically significant (P<0.05) differences in peak strength, rise time, time to peak and mean transit time among the various treatment groups. Changes in tumour blood perfusion were quantified by the assessment of contrast-enhanced ultrasound parameters. The results indirectly reflected the degree of change in angiogenesis in the tumour following experimental intervention. Ultrasound contrast imaging effectively showed the extent of the changes in vascularity and flow state. Therefore, contrast-enhanced ultrasound is suitable for use as an indicator of blood flow changes in an experimental model.
Highlights
A previous study demonstrated that Endostar improves the efficacy of radiotherapy in cell and animal experiments [1]
The present study aimed to investigate whether normalised vessels strengthen the role of radiation therapy of tumours by improving tumour tissue perfusion, thereby reducing the tumour hypoxia
By accurately determining the regions of interest (ROI) of the lesion, normal tissue and control area in an image using quantitative analysis software, the correct parameter measurements were ensured in the final analysis [9]
Summary
A previous study demonstrated that Endostar (recombinant human endostatin) improves the efficacy of radiotherapy in cell and animal experiments [1]. Endostar, which has a synergistic effect with radiotherapy, may act by inhibiting radiation‐induced hypoxia‐inducible factor‐1α and vascular endothelial growth factor, as well as by upregulating aquaporin 1 expression [2]. Endostar combined with radiotherapy significantly inhibits the growth of Lewis lung cancer and may affect tumour angiogenesis. The present study aimed to investigate whether normalised vessels strengthen the role of radiation therapy of tumours by improving tumour tissue perfusion, thereby reducing the tumour hypoxia. Intravenous injections of SonoVue, a second‐generation contrast agent comprising microbubbles of sulfur hexafluoride, are widely used for abdominal and superficial organ tumour imaging [4]. The echo of the contrast agent in the blood is uniform and pseudomorphism is not produced [5]
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