Continuous quantitative preparation methods of chromosome of peripheral blood lymphocytes

Abstract

Objective In order to provide theoretical basis for the standardization of chromosome preparation, we improve the efficiency of nuclear analysis by rapidly preparing the peripheral blood lymphocyte. Methods continuous quantitative method is optimized in the fixed time, baking and colchicine concentration, removing three room placed times fixed time based on the traditional preparation methods, Baking pieces immediately after the drop and improving the colchicine concentration to 0.008 μg/mL, compared with the traditional preparation method. Results Results of 278 chromosome specimens: 63% of the traditional group of 400~600, and 65% of continuous quantification group (P=0.065); The dispersion of chromosomes in the traditional group was 65%, and the good rate of the continuous quantification group was 66% (P=0.088). There was no significant difference between the two groups, indicating that there was no difference between two methods. Conclusion continuous quantitative of peripheral blood lymphocyte chromosome preparation method had good repeatability and consistency, shorten the chromosome preparation process and improved the efficiency of the chromosome analysis. Key words: Peripheral blood chromosome; Chromosome preparation; Continuous; Analysis of the efficiency; Quantitative