Continuous input DNA Nanomachine to program automated tumor diagnosis and treatment system

Abstract

The diagnosis of a single disease marker can provide important information about the occurrence and progression of the disease, but a single marker may not be sufficient to identify a certain type of disease and lead to false-positive results. Molecular logic gates provide smart options for simultaneous detection of biomarkers. In order to diagnose early-stage cancer with high sensitivity and directly perform automated precision treatment on identified cancer cells. In this study, all identification and computation modules were integrated into a single structure to construct a Pd@Pt ONPs-DNA nanomachine logic gate nanodevice, which enables specific recognition of three overexpressed cancer cell biomarkers. When any of the biomarkers are overexpressed, the OR logic circuit outputs a fluorescent signal, indicating the occurrence of a positive event. At the same time, the resulting fluorescence signal is then fed directly into the AND logic gate, when the input is (1, 1, 1), a laser output of 808 nm is generated. The photothermal properties and nanozyme activity of Pd@Pt ONPs convert the 808 nm laser into cytotoxicity, using continuously connected OR and AND gates for early warning of cancer and automatic and precise treatment.