Abstract
Nonfrozen and previously frozen red blood cells were washed by continuous‐flow centrifugation in reusable, stainless steel bowls. Satisfactory washout of extracellular Evans blue dye and intracellular glycerol from the red blood cell suspension was achieved, but complete mixing in the washing bowl was not accomplished.We studied the effects of the concentration of glycerol in the red cell suspension, the temperature (20 C or 40 C), spindle speed, flow rate, and composition of the electrolyte solution on the efficiency of washing in the reusable bowl. With this information in hand, we established automated programmed wash cycles.
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