Abstract
This study investigated the performance of different mixed microbial cultures (MMC) able to ferment crude glycerol generated from animal fat-based biodiesel to produce 1,3 propanediol (1,3 PDO) and butyric acid, under non-sterile conditions. Eight different continuous flow stirred-tank reactors (CSTR) were set up with different inoculum types and growth media. The distribution of metabolic products under variable operating conditions was determined. All MMC were characterized from a kinetic point of view and overall stoichiometric reactions were constructed. Changes in the microbial communities were monitored by means of Next Generation Sequencing (NGS). Maximum substrate degradation rate reached approximately 110g/L/d of glycerol (with a productivity of 38g/L/d and 11g/L/d for 1,3 PDO and butyric acid, respectively), obtained with an hydraulic retention time of 12h and 60g/L feed. The maximum feed concentration reached almost 90g/L, leading though to an incomplete substrate degradation.
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