Abstract
This study evaluated the propionic acid (HPr) production from crude glycerol (CG) (5000mgL-1) in an anaerobic fluidized bed reactor (AFBR). Grounded tire particles (2.8-3.35mm) were used as support material for microbial adhesion. The reactor was operated with hydraulic retention times (HRT) varying from 8 to 0.5h under mesophilic (30°C) conditions. The HPr was the main metabolite produced, increasing in composition from 66.5 to 99.6% by decreasing the HRT from 8 to 0.5h. Other metabolic products were 1,3-propanediol, with a maximum of 29.4% with an HRT of 6h, ethanol, acetic, and butyric acids. The decrease in HRT from 8 to 0.5h decreased the HPr yield, with a maximum of 0.48 ± 0.06g HPr g COD-1 and an HRT of 6h, and favored HPr productivity, with a maximum of 4.09 ± 1.24gL-1h-1 and HRT of 0.5h. In the biogas, the H2 content increased from 12.5 to 81.2% by decreasing the HRT from 8 to 0.5h. These results indicate the potential application of the AFBR for HPr production using an immobilized mixed culture.
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