Abstract
Hydrogen production from starch wastewater via sequential dark-photo fermentation process was investigated. Two anaerobic baffled reactors (ABRs) were operated in parallel at an OLR of 8.11±0.97g-COD/L/d, and a HRT of 15h. ABR-1 and ABR-2 was inoculated with pre-treated sludge and sludge immobilized on maghemite nanoparticles, respectively. Better hydrogen yield of 104.75±12.39mL-H2/g-COD-removed was achieved in ABR-2 as compared to 66.22±4.88mL-H2/g-COD-removed in ABR-1. The effluent of ABR-2 was used for further hydrogen production by photo fermentation in ABR-3. An overall hydrogen yield of 166.83±27.79mL-H2/g-COD-removed was achieved at a total HRT of 30h. 16S rRNA phylogeny showed that Clostridium and Rhodopseudomonas palustris species were dominant in ABR-1, ABR-2 and ABR-3, respectively.
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