Abstract
Changes in free (S) and bound polyamine contents were investigated in both the embryonic axes attached to the cotyledons and the embryonic axes excised from the whole chick-pea seeds before germination (isolated embryonic axes) during the first 24 hours at 25 °C, 30 °C or 35 °C. We also studied the distribution of these polyamines (PAs) along the embryonic axis of seeds germinated for 65 hours at 25 °C and 30 °C. The content in (S) polyamines was in general higher in embryonic axes separated from whole seeds than in isolated axes, suggesting a transport of these PAs from the cotyledons to the axis. At supraoptimal temperatures (S) polyamines decreased as germination proceeded, reaching higher levels than under the optimum temperature and the highest Spd and Spm accumulations were at 30 °C during the first 12 hours. The absence of cotyledons induced an important linkage of putrescine (Put) that strongly decrease with temperature. Methylglyoxalbis-(guanyl hydrazone) (MGBG) did not modify the total values of (S) and conjugated acid soluble (SH) polyamines, although it d change their distribution and increased conjugated acid insoluble (PH) polyamines.
Published Version
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