Consumption Safety Level of Carbamate, Organo Phosphate,Triazole,Neonicotinoid Pesticide Residues in the Fresh Vegetable Circulating in Bogor According to Analysis of Liquid Cromatography Spectrum Mass

Abstract

ABSTRACT Misde Yola *, Patimah *,Mica Tri Kumala Swandari** *Lecturer at Department of Pharmacy and Food Analysis, Health Polytechnic of the Ministry of Health, Jakarta II ** Lecturer at Department of Pharmacy, Al-Islami College of Health Sciences Al-Itihad, Cilacap, Central Java email: misdeyola@gmail.com Purpose to determine carbamate , organophosphate, triazole, neonicotinoid pesticide residues in fresh vegetables are still safe for human consumption with Methods Analysis of carbamate residues such as carbofuran and malathion such as organophosphate and prefenofos, imidaclopid, difeconazole , acephate suc a single pesticide in fresh vegetables mustard, cucumber and cabbage. Vegetables come from traditional markets in the Bogor area. Before being analyzed the three vegetables were washed with mineral water, warm water at 60oC, kitchen salt water and vegetables before washing. The vegetables were homogeneous with a homo mixer blender at 800rpm and after that extracted with acetonitrile with vortex quenchers extraction kit (EN method) for 5 minutes, centrifuge for 5 minutes at 6000rpm speed, supernatant filter with PTFE 13mmx0.22, then input 1 ml supernatant vortex for 5 minutes, centrifuge for 5 minutes at 6000rpm speed, supernatant filter with PTFE 13mmx0.22, then input 1 ml supernatant filtered into LC vials, injection with LCMSMS. Result none of the vegetables analyzed were positive for carbofuran and prefenofos. Conclusion: Consumption of fresh vegetables weighing 20 g / day is still safe for consumption Keywords: LC-MS / MS., Carbamate., organophosphate, a single pesticide