Abstract
Objective To evaluate the method and outcome of mixed human vascular ceils seeding on decellularized bovine pericardium in constructing a tissue engineered heart valves (TEHVs). Methods The mixed human vascular cells obtained by primary co-culture were seeded on decellularized bovine pericardium to construct a TEHVs. The morphologic and immunohistochemistry changes of the seeding cells, the ultramicrostructure of the decellularized bovine pericardium seeded with mixed human vascular cells were observed dynamically. Results The seeded cells overlaid on the deeellularized bovine pericardium gradually, and with the time-lapse, the cells stratified and penetrated into the deep layer of the pericardium. The mixed human vascular cells were verified as endothelial cells (ECs), smooth warble cells (SMC) and fibroblasts by the immunohistochemistry. And the seeding cells were testified for their ability of secreting t-PA and PAI-1 by enzyme-labeled immunosorbent assay (ELISA). The seeding cells on the pericardium were flat, lamellated or inordinate, and then stratified, with some floss and fibre. Conclusion The histocompatibility of the mixed human vascular cells and the decellularized bovine pericardium is satisfactory. The cells can grow well on the pericardium and possessed some functions of ECs. Key words: Tissue engineering; Heart valve prosthesis; Decellularize; Bovine pericardium
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
