Abstract
This work studied a novel method to construct a mixed reverse micelle system and its application in extracting and purifying enzymes. Mixed reversed micellar extraction formed by biosurfactant surfactin and ionic surfactant polysorbate-80 was applied for extracting cellulase. 40% surfactin and 60% polysorbate-80 were found to be the best system. The activity and protein recovery were obtained with the best conditions: in forward extraction, surfactant concentration was 3.0 mM; pH 7.0; temperature 40°C; KCl 0.05 M. In backward extraction, pH 7.5; temperature 40°C; KCl 0.1 M.
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