Abstract
Herein, a signal output SERS aptasensor for Histamine (HA) detection is designed. MIL-100(Fe) was loaded with gold nanoparticles (AuNPs) to form composite nanozyme MIL-100(Fe)@AuNPs, which was used in the reaction system TMB/H2O2. Silver nanoparticles (AgNPs) were synthesized as “amplifier” for the SERS signal of ox TMB. After nucleic acid functionalization, the two parts were assembled to form the multifunctional substrate with both high catalytic and SERS efficiency. In the detection system, the specific binding effect of HA aptamer toward HA induced a decrease in the assembly of AgNPs on MIL-100(Fe)@AuNPs which caused a decrease in ox TMB SERS signals. The linear relation of HA ranged from 10−11 M to 5 × 10−3 M with LOD as low as 3.9 × 10−12 M. Recovery ratio in fermented soybean products (94.42–105.75 %) proved the real sample applicability. The fabricated SERS aptasensor will provide technical support for the safety during food processing and storage.
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