Abstract
Objective To construct recombinant adenovirus vectors in which the cytosine deaminase (CD) gene is under the control of the human telomerase reverse transcriptase (hTERT) promoter. Methods Recombined adenovirus of Ad-hTERTp-CD was constructed using homologous recombination in cells by transforming shuttle vector pSU-Tp-CD and adenovirus backbone vector pBHGE3 into 293 cells. Results Recombinant adenovirus vectors were confirmed by PCR analysis and DNA sequencing. Conclusion Replication-defective adenovirus vector in which the CD gene was driven by hTERT promoter was made successfully. Key words: human telomerase reverse transcriptase (hTERT); cytosine deaminase gene (CD gene); adenovirus vector
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