Abstract
Dehydrins, one of the Late Embryogenesis Abundant (LEA) proteins, are specialized proteins related to environmental stress tolerance in plants. They are multifunctional proteins that can bind free metal ions, interact with macromolecules and specific membrane regions or participate in reduction of reactive oxygen species. Here, we report preparation of a plant transformation vector containing Arabidopsis dehydrin gene At1g54410 under the control of the strong constitutive double dCAMV 35S promoter and the selectable marker neomycin phosphotransferase gene driven by the nopaline synthase promoter. The gene At1g54410 was isolated from Arabidopsis genomic DNA by PCR approach. The resulting binary vector pND3 was introduced into the Agrobacterium tumefaciens strain LBA 4404 and verified for its stability in agrobacterial cells.
Highlights
Plants during their life cycle are constantly exposed to various environmental stresses such as drought, high rate of salinity, low temperatures and contamination by heavy metals which negatively affect plant survival and reduce agricultural yields
The gene At1g54410 was isolated from Arabidopsis genomic DNA by PCR approach
The expression of the dehydrin RcDhn5 from Rhododendron catawbiense contributed to freezing tolerance of transgenic Arabidopsis thaliana plants (Peng et al, 2008) or the expression of the dehydrin BjDHN2 from Brassica juncea increased heavy metal tolerance of transgenic plants Nicotiana tabacum (Xu et al, 2008)
Summary
Plants during their life cycle are constantly exposed to various environmental stresses such as drought, high rate of salinity, low temperatures and contamination by heavy metals which negatively affect plant survival and reduce agricultural yields To survive in such conditions, plants have to flexible respond by extensive changes of gene expressions, in biochemical signalling pathways and proteomic machinery (Ahuja et al, 2010). It was supposed that dehydrins containing histidine (His) rich regions may be involved in reduction of cellular damage during stress (Svensson et al, 2000; Hara et al, 2005) The structures such as His-X3-His and His-His may play role in buffering, serve as sensors of the level of free metal ions (Hara, 2010) or may reduce reactive oxygen species (ROS) (Hara et al, 2013). The stability of the plasmid in agrobacterial cells was confirmed by restriction analysis after re-transformation of pND3 into Escherichia coli
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