Abstract
RFLPs in Mitochondrial (mt) DNAs were analyzed using 21 strains from seven Fusarium species that cause fusarium head blight in wheat, including F. graminearum, F. culmorum, F. avenaceum, F. acuminatum, F. sporotrichioides, F. nivale, and F. tricinctum. MtDNA was purified by CsCl ultracentrifugation in the presence of bisbenzimide. Molecular sizes of mtDNAs were determined after digestion with restriction enzymes. A large interspecific variation was observed in mtDNA size: the largest one was 116 Kb in strain ARC-2125-1 of F. culmorum, while the smallest one was 49 kb in strain SUF-1121 of F. avenaceum. A considerable intraspecific size variation was also detected. All the species were identified by their mtDNA restriction patterns and assigned to individual clusters by UPGMA analysis, except for one strain of F. sporotrichioides. A Pstl clone library was constructed from strain KU-1615 of F. graminearum that is highly pathogenic and produces a large amount of nivalenol. Using this library, physical maps of F. gramineomm and F. culmorum were constructed. The mtDNA library and physical maps would facilitate species identification and further study of mtDNA polymorphisms in Fusarium species.
Published Version
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