Abstract
Objective To construct the neuregulin 1 (NRG1)-small interference RNA (siRNA) lentivirus vector.Methods NRG1-siRNA lentivirus vector was constructed by transfecting lentivirus vector GV123 with interfering sequences for NRG1 gene.The recombinant plasmid was packed and concentrated.The titer of lentivirus vector was measured in 293T cells.Then the astrocytes isolated and cultured in vitro were transfected with the lentivirus.NRG1 mRNA and protein expression was determined using RT-PCR and Western blot,respectively,72 h later.Results The NRG1-siRNA lentivirus vector GV123-siRNA was constructed successfully,and the titer of lentivirus vector was 8 × 108 TU/mL.The expression of NRG1 mRNA and protein was significantly down-regulated in the astrocytes after being transfected with the lentivirus vector.Conclusion The NRG1-siRNA lentivirus vector is constructed successfully. Key words: Neuregulin-1 ; RNA, small interfering; Lentivirus
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