Abstract
This paper describes a new formulation of magnetic nanoparticles coated by a novel polymer matrix----O-Carboxylmethylated Chitosan (O-CMC) as drug/gene carrier. The O-CMC magnetic nanoparticles were derivatized with a peptide obtained from the HIV-tat protein and transferrin to improve the translocation function and cellar uptake of the nanoparticles. To evaluate the O-MNPs-TAT-Tf system as drug carriers, Methotrexate (MTX) was incorporated as a model drug and MTX-loaded O-MNPs-TAT-Tf with an average diameter of 75nm were prepared and characterized by TEM, AFM and VSM. The cytotoxicity of MTX-loaded O-MNPs-TAT-Tf was investigated with C6 cells. The results showed that the MTX-loaded O-MNPs-TAT-Tf could be a novel magnetic targeting carrier. The ability of O-MNPs-Tat-Tf crossing BBB in rats was also investigated by single photon emission computed tomography (SPECT).
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call