Abstract
BackgroundCardiomyopathy is a progressive myocardial disorder. Here, we attempted to reveal the possible mechanism of cardiomyopathy at the transcription level with the roles of microRNAs (miRNAs) and transcription factors (TFs) taken into account.MethodWe firstly identified differentially expressed genes (DEGs) between cardiomyopathy patients and controls with data from the gene expression omnibus (GEO) database. DEGs were associated with the canonical pathways, molecular and cellular functions, physiological system development and function in the Ingenuity Knowledge Base by using the Ingenuity Pathway Analysis (IPA) software. TFs and miRNAs that DEGs significantly enriched were identified and a double-factor regulatory network was constructed.ResultsA total of 1,680 DEGs were identified. The DEGs were enriched for various pathways, with glucocorticoid receptor signaling as the most significant. A double-factor regulatory network was constructed, including seven TFs and two miRNAs. A subnetwork under the regulation of MEF2C and SRF was also constructed to illustrate their regulatory effects on cardiac functions.ConclusionOur results may provide new understanding of cardiomyopathy and may facilitate further therapeutic studies.
Highlights
The differentially expressed genes (DEG) were enriched for various pathways, with glucocorticoid receptor signaling as the most significant
The majority of the previous studies mainly focused on the analyses of differentially expressed genes (DEGs), without considering microRNAs and transcription factors (TFs) that regulate the expression of DEGs. miRNAs are small non-coding RNAs that control various biological processes through affecting
Molecular and cellular functions analysis revealed that ‘Cell Death and Survival’ (P =2.53 × 10−24 to 1.36 × 10−3) was the top molecular function affected by DEGs followed by ‘Cellular Growth and Proliferation’ (P =3.37 × 10−24 to 1.36 × 10−3) (Table 1)
Summary
We attempted to reveal the possible mechanism of cardiomyopathy at the transcription level with the roles of microRNAs (miRNAs) and transcription factors (TFs) taken into account. Cardiomyopathy is a progressive myocardial disorder, usually leading to cardiovascular death or heart failure-related disability [1]. It develops at any age, in either sex, and in any population [2,3]. In the current study, based on gene expression data from the Gene Expression Omnibus (GEO) database, we acquired DEGs, miRNAs and TFs that enriched with target DEGs and constructed a double-factor regulatory network. The majority of the previous studies mainly focused on the analyses of differentially expressed genes (DEGs), without considering microRNAs (miRNAs) and transcription factors (TFs) that regulate the expression of DEGs. miRNAs are small non-coding RNAs that control various biological processes through affecting
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