Construction of Lactobacillus casei-loaded Water-in-oil High Internal Phase Emulsion and its Study on Anti-breast Cancer Properties

Abstract

Objective: To prepare an oil-in-water high internal phase emulsion containing Lactobacillus casei, and to optimize and characterize the preparation process. To explore the therapeutic effect of the probiotic-carrying emulsion on in situ breast cancer in mice. Methods: Using corn oil, sodium alginate, polyglycerin ricinoleate and L. casei as raw materials, the high internal phase emulsion containing probiotic oil-in-water was prepared by high-speed homogenization method. The optimal preparation process was obtained through single factor investigation, microstructure characterization, stability investigation and in vitro simulation digestion experiment. Ten female BALB/c mice (SPF grade, 18–20 g) were selected. After the in situ breast cancer model was successfully established, the mice were randomly divided into experimental group and control group, with 5 mice in each group. The mice were administrated with probiotic-loaded emulsion and normal saline respectively, and were administrated with the stomach every other day for 10 days. The change of tumor volume during treatment and tumor mass at the end of treatment were recorded. Results: The optimum process of emulsion was: With the concentration of 5% Polyglycerin ricinolate, 75% aqueous volume, 3000r/min homogenizing speed, 30 s homogenizing time and 2% aqueous sodium alginate concentration, a high internal phase emulsion with a particle size of 9.33 ± 1.74 μm and an appearance of milky paste was prepared. The inclusion rate of probiotics reached 90.97 ± 27.09%. Breast cancer anti-tumor experiment showed that the tumor inhibition rate of the experimental group reached 33.78% compared with the control group. Conclusion: Water-in-oil high internal phase emulsion can effectively protect L. casei and reduce the loss of live probiotic when probiotics pass through the digestive fluid environment. Oral intragastric high internal phase emulsion containing L. casei oil-in-water has a certain therapeutic effect on breast cancer.