Construction of Biosensor for Detection of Phenolic Compound Using Thermostabilized Agaricus bisporus Tyrosinase

Abstract

Tyrosinase is a copper-containing enzyme that catalyzes the oxidation of phenols which is wide spread in nature from microorganisms to plants and animals. Tyrosinase was extracted from Agaricus bisporus, and its thermostability was studied at high temperature by using different polymer additives such as starch, agar and sucrose. The stability of the enzyme was enhanced with starch (10 %), agar (0.5 %) and sucrose (10 %). Enzyme stability in the presence of starch has been increased to \({111.2 ^{\circ}{\rm C}}\) which was directly recorded as a melting process by differential scanning calorimetry technique and also showed the considerable retention in the enzymatic activity up to 67 % when compared to the control. Voltammetric biosensor was constructed as a tool used to detect the phenolic compounds in a sample, for which the stabilized tyrosinase was fabricated on an electrochemical transducer. The constructed voltammetric thermostabilized tyrosinase biosensor can be used for the detection of phenolic compounds present in the environmental samples at extreme temperature conditions.