Abstract
This chapter discusses the potential usefulness of ionic liquids with respect to biocatalysis by illustrating the stability and activity of enzymes in ionic liquids in the presence or absence of water. Ionic liquids are a class of coulombic fluids composed of organic cations like alkyl-substituted imidazolium, pyrrolidinium, and tetraalkylammonium ions and anions such as halides, tetrafluoroborates, hexafluorophosphates, tosylates, etc. The possibility of tunable solvent properties by alternation of cations and anions has made ionic liquids attractive to study biocatalysis which warrants an understanding of enzyme stability and activity in ionic liquids. This chapter systematically outlines the recent studies on the stability of enzymes and their reactivity toward a wide range of catalytic reactions. A careful approach has been taken toward analysis of relationship between stability/activity of enzymes versus chaotropic/kosmotropic nature of cations and anions of ionic liquids.
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