Abstract
Objective: To construct a high-content imaging system analysis model to study the effects of the traditional Chinese medicine compounds puerarin on proliferation and migration of cervical cancer (HeLa) cells. Methods: The digital phase-contrast mode unique to the high-content imaging system was used to study the proliferation and migration effects of puerarin on HeLa cells. Results: Puerarin inhibited HeLa cell proliferation and migration. The high-content analysis model constructed in this study enabled efficiently and accurately determining cell proliferation and migration parameters in batches and allowed detecting multiple phenotypic parameters in one experiment. These parameters provide a theoretical basis for subsequent research. Conclusion: Puerarin inhibited HeLa cell proliferation and migration. The high-content analysis model constructed in this study uses a digital phase-contrast mode rather than traditional fluorescence staining and cell scratch tests for label-free analysis, thus providing a more rapid, efficient and accurate analysis method for tumor research.
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