Non-receptor tyrosine kinase Etk/BMX promotes migration and invasion of cervical cancer cells

Abstract

Objective To detect the effect of non-receptor tyrosine kinase Etk/BMX on migration and invasion of cervical cancer HeLa, SiHa and C-33A cells. Methods BMX-overexpression plasmids were transfected into C-33A cells to get stable C-33A-BMX overexpression clones; BMX-TALEN plasmids were transfected into HeLa cells to get stable knockout clones; shRNA BMX plasmids were transfected into SiHa cells to get stable BMX knockdown clones; wound healing assay was used to detect the effect of BMX on the migration of cervical cancer HeLa, SiHa and C-33A cells; transwell test was used to further detect the effect of BMX on the migration (without matrigel) and invasion (with matrigel). Results The results of wound healing assay showed that BMX overexpression promoted the migration of C-33A cells (P<0.05); The results of wound healing assay and transwell test showed that knockout or knockdown of BMX inhibited the migration and invasion of HeLa and SiHa cells significantly (P<0.05), at the same time, up-regulated expression of E-cadherin and down-regulated expression of N-cadherin were observed in BMX knockout or knockdown group. Conclusions BMX could promote the migration and invasion of cervical cancer cells. Key words: BMX; Cervical cancer cells; Migration; Invasion