Abstract
Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. ‘Morex’ was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX).
Highlights
Background & SummaryBarley (Hordeum vulgare L.) is a cereal grass of great agronomical importance
The last stage on the road towards the reference genome is the shotgun sequencing of bacterial artificial chromosome (BAC) clones along a minimum tiling path of the genome defined by the physical map
Sequencing of minimal tiling path (MTP)-BACs was conducted in four laboratories (Leibniz Institute on Aging—Fritz Lipmann Institute (FLI) Jena, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Beijing Genomics Institute (BGI) and Earlham Institute (EI) Norwich)
Summary
The goal of the International Barley Genome Sequencing Consortium (IBSC) is the construction of a map-based reference sequence assembly of barley cultivar ‘Morex’ by means of hierarchical shotgun sequencing[1] Towards this aim, the barley genomics community has developed an array of genome-wide physical and genetic mapping resources. We give (i) a comprehensive overview of datasets used for assembling the barley genome and methods employed in their generation, (ii) a detailed description of wet-lab procedures for BAC sequencing and the bioinformatics workflow of the sequence assembly and data integration procedures together with an outline of (iii) their browsable presentation in an online database These resources document the construction of the map-based reference sequence of the barley genome and will enable researchers to inspect the evidence used to assemble, order and orient sequence scaffolds and may guide the further improvement of the genome sequence with complementary data sets
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
